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外源性神经生长因子对内源性神经生长因子的影响

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外源性神经生长因子对内源性神经生长因子的影响 
雷正旺,洪光祥,王发斌

  目的:探讨外源性神经生长因子对内源性神经生长因子的影响。方法:采用大鼠坐骨神经硅管内再生室模型,靶器官内注射神经生长因子(实验组)或生理盐水 (对照组)2周。注射后1天、1周及2周,采用新生大鼠背根神经节细胞培养,测定硅管内液神经营养活性,用化学发光免疫检测法 (chemilumi-nescenceimmunoassay,CLIA)测定神经生长因子含量。结果:实验组与对照组细胞存活数及神经生长因子含量, 其差异有极显著性意义(t=6.49、6.42、8.82,P<0.01)。结论:靶器官注射外源性神经生长因子可以提高神经损伤处神经生长因子的含量及 活性
【作者单位】:武汉同济医科大学附属协和医院手外科
【关键词】:周围神经,神经生长因子,神经再生,神经营养
【分类号】:R322.85
【DOI】:cnki:ISSN:1005-054X.0.1997-01-004
【正文快照】:
  外源性神经生长因子对内源性神经生长因子的影响雷正旺洪光祥王发斌摘要目的:探讨外源性神经生长因子对内源性神经生长因子的影响。方法:采用大鼠坐骨神 经硅管内再生室模型,靶器官内注射神经生长因子(实验组)或生理盐水(对照组)2周。注射后1天、1周及2周,采用新生大鼠背根神经节细胞培养,测定硅管 内液神经营养活性,用化学发光免疫检测法(chemilumi-nescenceimmunoassay,CLIA)测定神经生长因子含量。结果:实验组 与对照组细胞存活数及神经生长因子含量,其差异有极显著性意义(t=6.4…

       Influence of exogenous nerve growth factor on endogenous nerve growth factor
  Lei Zhengwang;Hong Guangxiang;Wang Fabing. Department of Hand Surgery;Union Hospital;Tongji Medical University;Wuhan 430022
   Objective: To investigate the influence of exognous nerve growth factor (NGF) on endogenous NGF. Methods: A silicone chamber mode for sciatic nerve regeneration in adult rats had been used. The rats were given daily injections of exogenous NGF(experimental group) or normal saline solution(control group) to the target organ for 2 weeks. After 1 day、1 week and 2 weeks,we determined the neurotrophic activities by using neonatal rat dorsal root ganglia (DRG) neuron culture and measured the NGF concentrations by chemiluminescence immunoassay (CLIA) in the fluids accumulated in the silicone nerve regeneration chambers. Results:The numbers of surviving neurons and NGF concentrations of NGF treated animals are significantly different from those of controls( t=6.49、6.42、8.82,P<0.01) . Conclusions:Adminstration of NGF by target organ increased NGF neurotrophic activities and NGF content of injured nerve site.
【Keyword】:Peripheral nerve Nerve growth factor Nerve regeneration Reflex sympathetic dystrophy

                                                                                                      

 《中华手外科杂志》1997年01期